flow cytometry data acquisition system azurite  (DarklingX LLC)

Journal: The Journal of Biological Chemistry

Article Title: A subclass of serum anti-ZnT8 antibodies directed to the surface of live pancreatic β-cells

doi: 10.1074/jbc.RA117.000195

Figure Lengend Snippet: ZnT8-specific surface labeling of live INS-1E cells. A, representative confocal images of immunofluorescence staining of WT or ZnT8 KO cells using a proteoliposome- or liposome-immunized serum as indicated. Scale bar, 20 μm. B, histograms of surface immunofluorescence intensities measured in >10,000 WT INS-1E cells that were stained by a proteoliposome- and liposome-immunized serum as indicated. The solid lines are least square fits to a Lorentzian distribution. Arrows indicate serum titrations with increasing concentrations. C, identical experiments in B except that ZnT8 KO INS-1E cells were used. D, saturation of surface immunofluorescence staining with increasing serum concentrations. The concentration-intensity relationships were plotted using flow cytometry data in B and C (mean intensity ± S.E.). The solid lines are hyperbolic fits of concentration-dependent surface staining with a proteoliposome- or liposome-immunized serum as indicated.

Article Snippet: We thank Dr. Hao Zhang from the Flow Cytometry and Immunology Core Facility at Johns Hopkins Bloomberg School of Public Health for assistance in flow cytometry data acquisition and analysis.

Techniques: Labeling, Immunofluorescence, Staining, Concentration Assay, Flow Cytometry